Valorización de Garambullo en Síntesis Verde de Nanopartículas de Plata: Efecto de la Complejidad Fitoquímica y Capacidad Antioxidante Frente a Quercetina

Autores/as

  • María Guadalupe Lopéz Cortés
  • Ma. Cristina Irma Pérez-Pérez
  • José Amir González-Calderón
  • Gerardo Teniente-Martínez https://orcid.org/0000-0001-8649-845X
  • Sandra Herrera-Pérez
  • Susana Almanza-Rangel

DOI:

https://doi.org/10.29105/idcyta.v11i2.172

Palabras clave:

Actividad antimicrobiana, capacidad antioxidante, garambullo (Myrtillocactus geometrizans), nanopartículas de plata, síntesis verde

Resumen

Se evaluó el potencial del garambullo (Myrtillocactus geometrizans) como agente reductor y estabilizante en la síntesis verde de nanopartículas de plata (AgNPs), en comparación con quercetina comercial y de grado reactivo. El extracto presentó un contenido de compuestos fenólicos de 60.893 mg GAE/100 g, superior a reportes previos, lo que se reflejó en una elevada capacidad antioxidante (1559.11 µM TE/100 g por ABTS y 1016.50 µM TE/100 g por DPPH). La formación de AgNPs se confirmó mediante espectroscopía UV-Vis, observándose un plasmón característico alrededor de 430 nm, así como un cambio de color indicativo de la reducción de Ag⁺. Los sistemas con garambullo mostraron mayor estabilidad coloidal en el tiempo, mientras que los sistemas con quercetina, evidenciaron mayor tendencia a la aglomeración. En la evaluación antimicrobiana, las AgNPs sintetizadas con garambullo presentaron mayor actividad frente a Staphylococcus aureus ATCC-6538, con halos de inhibición de hasta 26.23±0.25 mm, mientras que en Escherichia coli ATCC-25922 la inhibición fue menor, 14.49±0.45 mm, atribuida a su estructura celular. Estos resultados evidencian que la complejidad fitoquímica del garambullo favorece tanto la síntesis como la estabilidad de las AgNPs, lo que destaca su potencial como alternativa sustentable para aplicaciones en el sector alimentario.

Descargas

Los datos de descargas todavía no están disponibles.

Citas

Abada, E., Mashraqi, A., Modafer, Y., Al Abboud, M. A., & El-Shabasy, A. (2024). Review: Green synthesis of silver nanoparticles by using plant extracts and their antimicrobial activity. Saudi Journal of Biological Sciences, 31(1), 103877. https://doi.org/10.1016/j.sjbs.2023.103877 DOI: https://doi.org/10.1016/j.sjbs.2023.103877

Arshad, F., Naikoo, G. A., Hassan, I. U., Chava, S. R., El-Tanani, M., Aljabali, A. A., & Tambuwala, M. M. (2024). Bioinspired and green synthesis of silver nanoparticles for medical applications: A green perspective. Applied Biochemistry and Biotechnology, 196, 3636–3669. https://doi.org/10.1007/s12010-023-04719-z DOI: https://doi.org/10.1007/s12010-023-04719-z

Badi’Ah, H. I., Seedeh, F., Supriyanto, G., & Zaidan, A. H. (2019). Synthesis of silver nanoparticles and the development in analysis method. IOP Conference Series: Earth and Environmental Science, 217(1), 012005. https://doi.org/10.1088/1755-1315/217/1/012005 DOI: https://doi.org/10.1088/1755-1315/217/1/012005

Bruna, T., Maldonado-Bravo, F., Jara, P., & Caro, N. (2021). Silver nanoparticles and their antibacterial applications. International Journal of Molecular Sciences, 22(13), 7202. https://doi.org/10.3390/ijms22137202 DOI: https://doi.org/10.3390/ijms22137202

Bubonja-Šonje, M., Knežević, S., & Abram, M. (2020). Challenges to antimicrobial susceptibility testing of plant-derived polyphenolic compounds. Archives of Industrial Hygiene and Toxicology, 71(4), 300–311. https://doi.org/10.2478/aiht-2020-71-3396 DOI: https://doi.org/10.2478/aiht-2020-71-3396

Grigoras, A. G., & Grigoras, V. C. (2024). Eco-friendly silver nanoparticles obtained by green synthesis from Salvia officinalis. Sustainable Chemistry, 5(3), 215–228. https://doi.org/10.3390/suschem5030014 DOI: https://doi.org/10.3390/suschem5030014

Gülcin, I. (2012). Antioxidant activity of food constituents: An overview. Archives of Toxicology, 86(3), 345–391. https://doi.org/10.1007/s00204-011-0774-2 DOI: https://doi.org/10.1007/s00204-011-0774-2

Huq, M. A., Ashrafudoulla, M., Rahman, M. M., Balusamy, S. R., & Akter, S. (2022). Green synthesis and potential antibacterial applications of bioactive silver nanoparticles: A review. Polymers, 14(4), 742. https://doi.org/10.3390/polym14040742 DOI: https://doi.org/10.3390/polym14040742

Ibrahim, N. H., Taha, G. M., Hagaggi, N. S. A., & Moghazy, M. A. (2024). Green synthesis of silver nanoparticles and its environmental sensor ability to some heavy metals. BMC Chemistry, 18, Article 7. https://doi.org/10.1186/s13065-023-01105-y DOI: https://doi.org/10.1186/s13065-023-01105-y

Jain, A. S., Pawar, P. S., Sarkar, A., Junnuthula, V., & Dyawanapelly, S. (2021). Bionanofactories for green synthesis of silver nanoparticles: Toward antimicrobial applications. International Journal of Molecular Sciences, 22(21), 11993. https://doi.org/10.3390/ijms222111993 DOI: https://doi.org/10.3390/ijms222111993

Jain, S., & Mehata, M. S. (2017). Medicinal plant leaf extract and pure flavonoid mediated green synthesis of silver nanoparticles and their enhanced antibacterial property. Scientific Reports, 7(1), 15867. https://doi.org/10.1038/s41598-017-15724-8 DOI: https://doi.org/10.1038/s41598-017-15724-8

López-Cortés, M. G., Peña-Juarez, M. G., Angel-Olarte, C. M. D., Delgado-Alvarado, E., Gonzalez-Calderon, J. A., & Pérez-Pérez, M. C. I. (2025). Green synthesis of silver nanoparticles using Myrtillocactus geometrizans extract for enhancing stability and antimicrobial properties. ChemistrySelect, 10(45), e03730. https://doi.org/10.1002/slct.202503730 DOI: https://doi.org/10.1002/slct.202503730

López-Palestina, C. U., Aguirre-Mancilla, C. L., Ramírez-Pimentel, J. G., Raya-Pérez, J. R., Santiago-Saenz, Y. O., Gutiérrez-Tlahque, J., & Hernández-Fuentes, A. (2019). Compuestos bioactivos y actividad antioxidante en tres estados de madurez de Myrtillocactus geometrizans provenientes del Valle del Mezquital, Hidalgo. Investigación y Desarrollo en Ciencia y Tecnología de Alimentos, 4(1), 317–322.

Pacheco, A. R. F., Cardoso, B. D., Pires, A., Pereira, A. M., Araújo, J. P., Carvalho, V. M., Rodrigues, R. O., Coutinho, P. J. G., Castelo-Grande, T., & Castanheira, E. M. S. (2023). Development of pH-sensitive magnetoliposomes containing shape anisotropic nanoparticles for potential application in combined cancer therapy. Nanomaterials, 13(6), 1051. https://doi.org/10.3390/nano13061051 DOI: https://doi.org/10.3390/nano13061051

Parmar, S., Kaur, H., Singh, J., Matharu, A. S., Ramakrishna, S., & Bechelany, M. (2022). Recent advances in green synthesis of Ag NPs for extenuating antimicrobial resistance. Nanomaterials, 12(7), 1115. https://doi.org/10.3390/nano12071115 DOI: https://doi.org/10.3390/nano12071115

Rabha, D., Sarma, H., Borah, M., Ghose, V., Barman, D., Rao, S., Sarma, H. S., Devi, R., & Chandra Boruah, D. (2024). Clerodendrum japonicum (Thunb.) Sweet leaf extract supported nanosilver particles: Characterization, antioxidant and antibacterial activity. Vietnam Journal of Science and Technology, 62(6), 1065–1076. https://doi.org/10.15625/2525-2518/19376 DOI: https://doi.org/10.15625/2525-2518/19376

Ramírez-Rosas, S. L., Delgado-Alvarado, E., Sánchez-Vargas, L. O., Herrera-May, A. L., Peña-Juárez, M. G., & González-Calderon, J. A. (2022). Green route to produce silver nanoparticles using the bioactive flavonoid quercetin as a reducing agent and food anti-caking agents as stabilizers. Nanomaterials, 12(19), 3545. https://doi.org/10.3390/nano12193545 DOI: https://doi.org/10.3390/nano12193545

Rizwana, H., Alwhibi, M. S., Aldarsone, H. A., Awad, M. A., Soliman, D. A., & Bhat, R. S. (2021). Green synthesis, characterization, and antimicrobial activity of silver nanoparticles prepared using Trigonella foenum-graecum L. leaves grown in Saudi Arabia. Green Processing and Synthesis, 10(1), 421–429. https://doi.org/10.1515/gps-2021-0043 DOI: https://doi.org/10.1515/gps-2021-0043

Rodrigues, A. S., Batista, J. G. S., Rodrigues, M. Á. V., Thipe, V. C., Minarini, L. A. R., Lopes, P. S., & Lugão, A. B. (2024). Advances in silver nanoparticles: A comprehensive review on their potential as antimicrobial agents and their mechanisms of action elucidated by proteomics. Frontiers in Microbiology, 15, 1440065. https://doi.org/10.3389/fmicb.2024.1440065 DOI: https://doi.org/10.3389/fmicb.2024.1440065

Samuggam, S., Chinni, S. V., Mutusamy, P., Gopinath, S. C., Anbu, P., Venugopal, V., Reddy, L. V., & Enugutti, B. (2021). Green synthesis and characterization of silver nanoparticles using Spondias mombin extract and their antimicrobial activity against biofilm-producing bacteria. Molecules, 26(9), 2681. https://doi.org/10.3390/molecules26092681 DOI: https://doi.org/10.3390/molecules26092681

Sánchez-Recillas, E., Campos-Vega, R., Pérez-Ramírez, I. F., Luzardo-Ocampo, I., Cuéllar-Núñez, M. L., & Vergara-Castañeda, H. A. (2022). Garambullo (Myrtillocactus geometrizans): Effect of in vitro gastrointestinal digestion on the bioaccessibility and antioxidant capacity of phytochemicals. Food & Function, 13(8), 4699–4713. https://doi.org/10.1039/D1FO04392G DOI: https://doi.org/10.1039/D1FO04392G

Shahzadi, S., Fatima, S., Shafiq, Z., & Janjua, M. R. S. A. (2025). Green synthesis of silver nanoparticles using plant extracts. RSC Advances, 15, 3858–3903. https://doi.org/10.1039/D4RA07519F DOI: https://doi.org/10.1039/D4RA07519F

Sharma, R., Basist, P., Alhalmi, A., Khan, R., Noman, O. M., & Alahdab, A. (2023). Synthesis of quercetin-loaded silver nanoparticles and assessing their anti-bacterial potential. Micromachines, 14(12), 2154. https://doi.org/10.3390/mi14122154 DOI: https://doi.org/10.3390/mi14122154

Singh, S., Arya, H., Sahu, W., Reddy, K. S., Nimesh, S., Alotaibi, B. S., & Kumar Bhatt, T. (2024). Green synthesized silver nanoparticles of Terminalia bellirica leaves extract: Synthesis, characterization, in-silico studies, and antimalarial activity. Artificial Cells, Nanomedicine, and Biotechnology, 52(1), 238–249. https://doi.org/10.1080/21691401.2024.2339429 DOI: https://doi.org/10.1080/21691401.2024.2339429

Singleton, V., Orthofer, R., & Lamuela-Raventós, R. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299, 152–178. https://doi.org/10.1016/S0076-6879(99)99017-1 DOI: https://doi.org/10.1016/S0076-6879(99)99017-1

Vanlalveni, C., Lallianrawna, S., Biswas, A., Selvaraj, M., Changmai, B., & Rokhum, S. L. (2021). Green synthesis of silver nanoparticles using plant extracts and their antimicrobial activities: A review of recent literature. RSC Advances, 11, 2804–2837. https://doi.org/10.1039/D0RA09941D DOI: https://doi.org/10.1039/D0RA09941D

Descargas

Publicado

2026-08-01

Cómo citar

Lopéz Cortés, M. G., Pérez-Pérez, M. C. I., González-Calderón, J. A., Teniente-Martínez, G., Herrera-Pérez, S., & Almanza-Rangel, S. (2026). Valorización de Garambullo en Síntesis Verde de Nanopartículas de Plata: Efecto de la Complejidad Fitoquímica y Capacidad Antioxidante Frente a Quercetina. Investigación Y Desarrollo En Ciencia Y Tecnología De Alimentos, 11(2), 54–64. https://doi.org/10.29105/idcyta.v11i2.172

Datos de los fondos