Desarrollo de un método de extracción por la técnica de MEPS para polifenoles en miel
DOI:
https://doi.org/10.29105/idcyta.v8i1.110Keywords:
Honey, polyphenols, MEPS, sorbent, optimizationAbstract
A method was developed and validated using the microextraction with packed sorbent (MEPS) technique for the extraction of polyphenols in honey and their analysis by high performance liquid chromatography. The C18 cartridge turned out to be the most suitable for the simultaneous extraction of a greater number of polyphenols, such as protocatechuic acid, caffeic acid, p-coumaric acid, trans-ferulic acid, rosmarinic acid, quercetin, luteolin, apigenin and kaempferol. To optimize the volume and type of solvent in the elution step, a design of experiments was used using the D-Optimal model, with 50 µL of 100% methanol being the appropriate condition for recovering the analytes. Under the conditions selected as optimal, the method turned out to be linear, precise and accurate with detection and quantification limits of 0.039 to 0.071 μg/mL and 0.131 to 0.238 μg/mL, respectively. The application of the method was carried out through the analysis of a honey sample, which turned out to be accurate, finding enrichment factors of 2.64 and 2.86 for kaempferol and apigenin, respectively.
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