Actividad citotóxica, antioxidante y antihemolítica del extracto metanólico de Cymbopogon citratus (DC.) Stapf
DOI:
https://doi.org/10.29105/idcyta.v8i1.121Keywords:
Cytotoxicity, Mexican medicinal plants, Cymbopogon citratus, LemongrassAbstract
Cymbopogon citratus is an aromatic plant widely used as an infusion worldwide that stands out for its pharmacological and biological effects. The aim of this paper is to investigate the cytotoxic, antioxidant, and antihemolytic activity of the methanolic extract of C. citratus. The extract was obtained by extraction with absolute methanol in Soxhlet equipment for 48 h; conventional phytochemical tests were performed; the cytotoxic activity was evaluated by MTT colorimetric technique at 570 nm. In addition, the percentage of antioxidant activity was evaluated by the DPPH (Abs = 517 nm) free radical assay and the ability to protect exposed erythrocytes against the azo radical oxidant (AAPH, Abs = 540 nm), which causes oxidation of erythrocyte membranes. The main natural products found in C. citratus extract were coumarins, sterols, and flavonoids. The extract possesses considerable cytotoxic activity against lymphoma line L5178Y-R (IC50 = 209.2 µg/ml), is not toxic in human erythrocytes (IC50 = 607.0 µg/ml), and shows a high erythrocyte protective capacity against AAPH (IC50 = 7.0 µg/ml). These results demonstrate the potential biological effects of C. citratus in in-vitro assays. Henceforth, our laboratory team will continue to work to isolate and identify the bioactive compounds from C. citratus extract with cytotoxic effects to find more alternatives for cancer treatment.
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Bazaldúa-Rodríguez, A. F. et al. (2021) ‘Furanocoumarins from Ruta chalepensis with Amebicide Activity’, Molecules, 26(12), p. 3684. doi: 10.3390/molecules26123684. DOI: https://doi.org/10.3390/molecules26123684
Cárdenas Garza, G. R. et al. (2021) ‘Benefits of Cardamom (Elettaria cardamomum (L.) Maton) and Turmeric (Curcuma longa L.) Extracts for Their Applications as Natural Anti-Inflammatory Adjuvants’, Plants, 10(9), p. 1908. doi: 10.3390/plants10091908. DOI: https://doi.org/10.3390/plants10091908
De La Cruz-Jiménez, L. et al. (2022) ‘Biological Activities of Seven Medicinal Plants Used in Chiapas, Mexico’, Plants, 11(14), p. 1790. doi: 10.3390/plants11141790. DOI: https://doi.org/10.3390/plants11141790
De Oliveira E Silva, F. et al. (2022) ‘Cymbopogon citratus Protects Erythrocytes from Lipid Peroxidation in vitro’, Cardiovascular & Hematological Agents in Medicinal Chemistry. Netherlands, 20(2), pp. 166–169. doi: 10.2174/1871525719666210906122948. DOI: https://doi.org/10.2174/1871525719666210906122948
Ekpenyong, C. E., Akpan, E. and Nyoh, A. (2015) ‘Ethnopharmacology, phytochemistry, and biological activities of Cymbopogon citratus (DC.) Stapf extracts’, Chinese Journal of Natural Medicines. China Pharmaceutical University, 13(5), pp. 321–337. doi: 10.1016/S1875-5364(15)30023-6. DOI: https://doi.org/10.1016/S1875-5364(15)30023-6
Elizondo-Luévano, J. H. et al. (2020) ‘Berberina, curcumina y quercetina como potenciales agentes con capacidad antiparasitaria’, Revista de Biología Tropical, 68(4), pp. 1241–1249. doi: 10.15517/rbt.v68i4.42094. DOI: https://doi.org/10.15517/rbt.v68i4.42094
Elizondo-Luévano, J. H. et al. (2022) ‘In Vitro Cytotoxic Activity of Methanol Extracts of Selected Medicinal Plants Traditionally Used in Mexico against Human Hepatocellular Carcinoma’, Plants, 11(21), p. 2862. doi: 10.3390/plants11212862. DOI: https://doi.org/10.3390/plants11212862
Gomez-Flores, R. et al. (2009) ‘Antitumor properties of Gymnosperma glutinosum leaf extracts.’, Cancer investigation, 27(2), pp. 149–55. doi: 10.1080/07357900802192190. DOI: https://doi.org/10.1080/07357900802192190
Guillén-Meléndez, G. A. et al. (2021) ‘Cytotoxic Effect In Vitro of Acalypha monostachya Extracts over Human Tumor Cell Lines’, Plants, 10(11), p. 2326. doi: 10.3390/plants10112326. DOI: https://doi.org/10.3390/plants10112326
López Villarreal, S. M. et al. (2022) ‘Preliminary Study of the Antimicrobial, Anticoagulant, Antioxidant, Cytotoxic, and Anti-Inflammatory Activity of Five Selected Plants with Therapeutic Application in Dentistry.’, International journal of environmental research and public health, 19(13), p. 7927. doi: 10.3390/ijerph19137927. DOI: https://doi.org/10.3390/ijerph19137927
Molina-Garza, Z. J. et al. (2014) ‘Anti-Trypanosoma cruzi activity of 10 medicinal plants used in northeast Mexico.’, Acta tropica. Elsevier B.V., 136(1), pp. 14–8. doi: 10.1016/j.actatropica.2014.04.006. DOI: https://doi.org/10.1016/j.actatropica.2014.04.006
Ngamwonglumlert, L., Devahastin, S. and Chiewchan, N. (2017) ‘Natural colorants: Pigment stability and extraction yield enhancement via utilization of appropriate pretreatment and extraction methods.’, Critical reviews in food science and nutrition, 57(15), pp. 3243–3259. doi: 10.1080/10408398.2015.1109498. DOI: https://doi.org/10.1080/10408398.2015.1109498
Quintanilla-Licea, R. et al. (2014) ‘Antiprotozoal activity against Entamoeba histolytica of plants used in northeast mexican traditional medicine. Bioactive compounds from Lippia graveolens and Ruta chalepensis’, Molecules, 19(12), pp. 21044–21065. doi: 10.3390/molecules191221044. DOI: https://doi.org/10.3390/molecules191221044
Quintanilla-Licea, R. et al. (2020) ‘Antiprotozoal Activity against Entamoeba histolytica of Flavonoids Isolated from Lippia graveolens Kunth.’, Molecules (Basel, Switzerland), 25(11), p. 2464. doi: 10.3390/molecules25112464. DOI: https://doi.org/10.3390/molecules25112464
Ramírez-Villalobos, J. M. et al. (2021) ‘In Vitro Tumor Cell Growth Inhibition Induced by Lophocereus marginatus (DC.) S. Arias and Terrazas Endophytic Fungi Extracts.’, International journal of environmental research and public health, 18(18), p. 9917. doi: 10.3390/ijerph18189917. DOI: https://doi.org/10.3390/ijerph18189917
Rodríguez-Garza, N. E. et al. (2023) ‘In Vitro Biological Activity and Lymphoma Cell Growth Inhibition by Selected Mexican Medicinal Plants’, Life, 13(4), p. 958. doi: 10.3390/life13040958. DOI: https://doi.org/10.3390/life13040958
Rodríguez-Magaña, M. P. et al. (2019) ‘Hypoglycemic Activity of Tilia americana, Borago officinalis, Chenopodium nuttalliae, and Piper sanctum on Wistar Rats’, Journal of Diabetes Research, 2019, p. 7836820. doi: 10.1155/2019/7836820. DOI: https://doi.org/10.1155/2019/7836820
Romero-Arguelles, R. et al. (2022) ‘In Vitro Antitumor Activity of Endophytic and Rhizosphere Gram-Positive Bacteria from Ibervillea sonorae (S. Watson) Greene against L5178Y-R Lymphoma Cells.’, International journal of environmental research and public health, 19(2), p. 894. doi: 10.3390/ijerph19020894. DOI: https://doi.org/10.3390/ijerph19020894
Vázquez-Briones, M. and Guerrero-Beltrán, J. (2017) ‘Effect of essential oil of Cymbopogon citratus in the physicochemical properties of quitosan films’, Scientia Agropecuaria, 8(4), pp. 401–409. doi: 10.17268/sci.agropecu.2017.04.11. DOI: https://doi.org/10.17268/sci.agropecu.2017.04.11
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Copyright (c) 2023 Ramiro Quintanilla-Licea , Nancy Edith Rodríguez-Garza, Ángel David Torres-Hernández, María Julia Verde-Star , Joel Horacio Elizondo-Luévano
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