Optimización de métodos de extracción de R-Ficoeritrina a partir de Porphyridium cruentum
DOI:
https://doi.org/10.29105/idcyta.v8i1.15Keywords:
Microalgae, optimization, purityAbstract
Microalgae are natural, valuable products with the potential to cover food needs. R-Phycoerythrin (R-PE) is a pigment obtained from the microalgae P. cruentum that has gained interest as a pink colorant in foods. Therefore, this research focused on optimizing extraction methods. Response Surface Methodology (RSM) was obtained with a central composite design and three central points to evaluate the influence of homogenization time, concentration and buffer volume in obtaining R-PE and a high purity index (PI). The methods evaluated were maceration, maceration with ultrasonic bath and combined maceration with an ultrasonic probe. The optimal conditions for maceration were 10 min of homogenization and 3 mL of 0.1 M buffer, for maceration with ultrasonic bath they were 5 min of homogenization with 3 mL of 0.1 M buffer and for maceration with ultrasonic probe they were 7.9 min of homogenization with 5.7 mL of 0.8 M buffer. The results showed the potential of R-PE extraction methods to be used as a natural dye.
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