Comparación de la recuperación de células de Salmonella adheridas al tomate Roma con el uso de ultrasonido y vortex
DOI:
https://doi.org/10.29105/idcyta.v8i1.17Keywords:
fruits, foodborne, pathogens, sampling, detachmentAbstract
Consumption of tomatoes has been associated with outbreaks of salmonellosis. The microorganisms present on the surface of the fruit can be strongly attached (SA), which makes their removal difficult. The objective of this work was to compare the recovery of SA from Salmonella in Roma tomato samples exposed to ultrasound or vortex, with three exposure times. Each tomato inoculated with rifampicin-resistant Salmonella was manually rinsed with 100 mL of buffered peptone water (BPW) to remove loosely attached cells. The tomato was submerged in 100 ml of BPW and exposed at ultrasonic bath (40 kHz) or vortexing (6 000 rpm), for 1, 5, and 10 min. Salmonella SA counts from tomatoes epicarp were (p < 0.05) different depending on the shaking method and time. The use of the vortex achieved a greater recovery of the pathogen compared to ultrasound. Lower SA counts were obtained with exposure for 1 min than with 5 and 10 min. Both methods were successful in recovering Salmonella CFA from tomato epicarp, it is important to consider agitation time effect on tomato integrity when choosing a method.
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