Antimicrobial activity of 3-hydroxypropionaldehyde produced by Limosilactobacillus reuteri cultivated in MRS broth replacing the carbon source
DOI:
https://doi.org/10.29105/idcyta.v11i2.175Keywords:
Limosilactobacillus reuteri, 3-Hydroxypropionaldehyde, Antimicrobial activity, MRS broth-Cello, MRS broth-GlucoseAbstract
The aim of this study was to determine the effect of 1% cellobiose as the main carbon source in an MRS culture of Limosilactobacillus reuteri, formulated for the biotransformation of glycerol to 3-hydroxypropanal, a broad-spectrum antimicrobial agent. Escherichia coli O157:H7 was used as a model organism to evaluate the effect of bacterial inhibition. A second MRS broth formulated with 1% glucose and a standard commercial MRS broth were used as comparatives. The 3-HPA used was produced during the exponential growth phase (2 to 12 hours) of each MRS culture. The 3-HPA produced in the media with cellobiose, and the commercial MRS broth inhibited 45.01±18.51% and 35.94 ± 7.76% (p>0.05) respectively of E. coli growth, while 8.50 ± 0.37% inhibition occurred with the 3-HPA produced in MRS culture with glucose. As a control antiseptic agent, 0.12% chlorhexidine was used, showing 97.61 ± 0.05% inhibition. It is concluded that the initial carbon source modulates the antimicrobial potential of 3-HPA, with cellobiose as a candidate substrate for further study to optimise active metabolite secretion, highlighting its importance in the development of alternatives for microbial control and the circular economy.
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